Fill volume for 1.7mm NMR tubes

The SSPPS NMR Facility routinely uses a 1.7mm micro-cryoprobe that takes capillary NMR tubes with a diameter of 1.7mm instead of the standard 5mm NMR tubes. These capillary tubes require much less sample volume than a standard tube, but exactly how much should be used? And what happens if there is too little? Or too much?

Linewidth titrations

NMR is one of the most powerful techniques to monitor molecular interactions because of the wealth of information it provides. The previous post described monitoring chemical shift changes during titrations, but chemical shifts are not the only NMR parameter that can change during titrations. Often linewidths change as well. By measuring linewidth changes the dissociation rate, k_off, may be determined.