Wednesday, December 2, 2020

DOSY processing

DOSY experiments can resolve signals from the individual components of a mixture of compounds without physically separating the compounds. This is achieved by using the different rates that molecules diffuse through solution to separate the signals. DOSY data are typically presented as a multi-dimensional NMR spectrum where one of the dimensions corresponds to the diffusion rate. Unlike the frequency dimensions, the diffusion data is not processed with a fourier transform. Instead, non-linear curve fitting is used to extract rates from which a pseudo spectrum is created.