NMR titrations are often used to demonstrate binding, locate active sites, and measure affinities. By measuring changes in chemical shifts or linewidths as the concentration of one of the binding partners is changed, quantitative data, such as dissociation constants and dissociation rates, may be obtained. In most cases the systems studied involve 1:1 binding. Systems with multiple binding sites are more difficult to analyse but, with some simplifying assumptions, quantitative information, such as stoichiometry, may still be obtained.
