Monday, May 2, 2022

NUS for COSY experiments

Non Uniform Sampling (NUS) reduces the time taken to acquire multi-dimensional NMR spectra by predicting a fraction of the normal data instead of measuring it. The most commonly used algorithm for reconstructing the missing data requires the collected data to be properly phased in the indirect dimension. For this reason I have not recommended using NUS with HMBC and gCOSY experiments. However, last month's post showed that unphaseable HMBC experiments cope with NUS just as well as the phaseable LR-HSQMBC. In this post I compare the unphaseable gCOSY experiment with the phaseable CLIP-COSY to see how they are impacted by NUS