NMR is one of the best methods for studying molecular interactions. To identify small molecules that bind to macromolecules many different experiments can be used, one of which is the waterLOGSY experiment. This is a 1D, 1H-detected experiment that can be run in five minutes and is best suited for weakly interacting complexes (mM KDs), a binding regime that is difficult to probe by other techniques. To demonstrate how it works I will show spectra of a mixture of L-tryptophan and D-sucrose in the presence and absence of bovine serum albumin.
